Visualising latent DNA on swabs
نویسندگان
چکیده
منابع مشابه
Survival of bacteria on swabs.
It has long been recognized that bacteria fail to survive for long periods on cotton swabs. Unsaturated fatty acids have been shown to inhibit a number of species (Pollock, Brit. J. Exptl. Pathol. 28:295, 1947; Rowatt, J. Gen. Microbiol. 17:279, 1957), and the presence of these substances in cotton has been demonstrated (Pollock, Nature 161:853, 1948). Various attempts have been made to overcom...
متن کاملBuccal DNA collection: comparison of buccal swabs with FTA cards.
Collection and analysis of DNA, most commonly from blood or buccal cells, is becoming more common in epidemiologic studies. Buccal samples, which are painless to take and relatively easily collected, are often the preferred source. There are several buccal cell collection methods: swabs, brushes, mouthwash, and treated cards, such as FTA or IsoCode cards. Few studies have systematically compare...
متن کاملVisualising individual sequence-specific protein-DNA interactions in situ.
Gene expression - a key feature for modulating cell fate-is regulated in part by histone modifications, which modulate accessibility of the chromatin to transcription factors. Until now, protein-DNA interactions (PDIs) have mostly been studied in bulk without retrieving spatial information from the sample or with poor sequence resolution. New tools are needed to reveal proteins interacting with...
متن کاملVaricella zoster virus DNA in throat swabs of vaccinees.
Ninety throat swabs from 30 healthy children who received varicella vaccine were tested for varicella zoster virus DNA by the polymerase chain reaction. Seroconversion was observed in 27 of 30 (90%) vaccinees. The positive rates were 7.4% (2/27) at one week and 0% (0/27) at four weeks after vaccination. These rates were considerably lower than those in normal children with varicella.
متن کاملDNA IQ Isolation of Genomic DNA from Stains and Buccal Swabs.
MATERIALS Reagents Dithiothreitol (DTT), 1 M DNA IQ System (Promega; includes Resin, Lysis Buffer, 2X Wash Buffer, and Elution Buffer) Ethanol, 95-100% Isopropanol Sample (liquid blood, cotton swab, th CEP swab, 15-50 mm2 S&S paper, 3-30 mm2 FTA paper) Equipment Aerosol-resistant micropipette tips DNA IQ Spin Baskets (Promega) Heating blocks, preset to 65°C and 95oC MagneSphere Technology Magne...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: Forensic Science International
سال: 2018
ISSN: 0379-0738
DOI: 10.1016/j.forsciint.2018.08.016